Chitotriosidase 1 antibody

Catalog No. ABIN204118

This Mouse Monoclonal antibody specifically detects Chitotriosidase 1 in ELISA, IP and Neut. It exhibits reactivity toward Human and Mouse.

Quick Overview for Chitotriosidase 1 antibody (ABIN204118)

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chitotriosidase 1 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Product Details anti-Chitotriosidase 1 Antibody

Specificity: Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhCHI-3L1. Species sequence homology: mouse: 79 % .

Purification: Protein G purified

Immunogen: NS0-derived rhCHIT-1.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IP (25 μg/mL), Neut

Usage: Suitable for use in ELISA, Immunoprecipitation and Neutralization. ELISA (direct): 0.5-1.0 μg/mL. Detection limit for rhCHIT1 is approximately 2 ng/well. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhCHIT1 from conditioned cell culture medium. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N, N', N''-triacetylchitotriose. 50 % of the enzymatic activity was inhibited by the antibody at approximately 56 ng/mL (IC 50) under conditions in which the enzyme was present at 20 ng/mL and the substrate concentration was 0.01  mM. IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Chitotriosidase 1

Target: Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))

Alternative Name: CHIT1 / Chitotriosidase

Background: Name/Gene ID: CHIT1

Synonyms: CHIT1, CHITD, CHI3, Chitinase-1, Chitinase 1 (chitotriosidase), Chitotriosidase, CHIT, Chitotriosidase-1

Gene ID: 1118

UniProt: Q13231