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Cytochrome C antibody

This Mouse Monoclonal antibody specifically detects Cytochrome C in IF, ELISA and IP. It exhibits reactivity toward Human, Rat and Mouse.
Catalog No. ABIN204163

Quick Overview for Cytochrome C antibody (ABIN204163)

Target

See all Cytochrome C (CYCS) Antibodies
Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity

  • 202
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  • 137
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  • 5
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  • 1
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Human, Rat, Mouse

Host

  • 112
  • 107
  • 5
  • 1
Mouse

Clonality

  • 137
  • 87
  • 1
Monoclonal

Conjugate

  • 115
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
This Cytochrome C antibody is un-conjugated

Application

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Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)
  • Specificity

    Recognizes native rat Cytochrome C, but does not recognize denatured Cytochrome C. Recognizes a region in the vicinity of aa62 of rat Cytochrome C. Species cross-reactivity: Human, mouse, rabbit and Drosophila. Not tested for Immunofluorescence in rabbit and Drosophila.

    Purification

    Protein A purified

    Immunogen

    Rat Cytochrome c.

    Type of Immunogen: Purified protein

    Isotype

    IgG1b kappa
  • Application Notes

    Approved: ELISA (0.1 - 1 μg/mL), IF (20 μg/mL), IP

    Usage: Suitable for use in Immunofluorescence, ELISA and Immunoprecipitation. ELISA (Native): 0.1-1 μg/mL. Immunofluorescence: 20 μg/mL. Immunoprecipitation (Native): 15 μg (for extract from ~1 x106). Positive control: HeLa, Rat6.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

    Alternative Name

    CYCS / Cytochrome c

    Background

    Name/Gene ID: CYCS

    Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

    Gene ID

    54205

    UniProt

    P99999

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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