NOS1 antibody (N-Term)

Catalog No. ABIN204492

This anti-NOS1 antibody is a Mouse Monoclonal antibody detecting NOS1 in WB, IHC, ELISA, IP and IHC (p). Suitable for Rat.

Quick Overview for NOS1 antibody (N-Term) (ABIN204492)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NOS1 Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~160kD nNOS. Reactivity has been confirmed with rat brain tissue lysates. The nNOS target band at ~160kD was detected by immunoprecipitation and Western blots. Immunohistochemical tests using human, mouse, and rat brain tissue sections yielded positive and specific cytoplasmic staining in neurons and astrocytes. Based on amino acid sequence homology, the antibody is expected to recognize mouse (95 % ) and human (81 %) nNOS.

Purification: Protein A purified

Immunogen: Recombinant protein derived from the N-terminal region of rat nNOS protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Western Blot: 1 μg/mL. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 7 μg/each. Immunohistochemistry (FFPE): 2 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1 / nNOS

Background: Name/Gene ID: NOS1

Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction