NOTCH2 antibody (AA 2063-2413)

Catalog No. ABIN204497

The Rat Monoclonal anti-NOTCH2 antibody has been validated for WB and ELISA. It is suitable to detect NOTCH2 in samples from Human.

Quick Overview for NOTCH2 antibody (AA 2063-2413) (ABIN204497)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NOTCH2 Antibody

Binding Specificity: AA 2063-2413

Specificity: Recognizes human Notch-2.

Purification: Protein G purified

Sterility: 0.2 μm filtered

Immunogen: E. coli-derived recombinant human Notch-2 ICD segment (aa2063-2413) Accession # Q04721.

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot: 1-2 μg/mL, With an appropriate colorimetric detection system, the detection limit for rhNotch-2 ICD is ~5 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 μg/mL, Detection limit for rhNotch-2 ICD is ~12 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL sterile, ddH2O or sterile 40-50 % glycerol.

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: NOTCH2 / NOTCH-2

Background: Name/Gene ID: NOTCH2

Synonyms: NOTCH2, AGS2, HN2, Notch (Drosophila) homolog 2, Notch homolog 2 (Drosophila), Notch 2, Notch homolog 2, HJCYS

Gene ID: 4853

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance