Occludin antibody (C-Term) (FITC)

Catalog No. ABIN204503

The Mouse Monoclonal anti-Occludin antibody has been validated for WB, ELISA and IF. It is suitable to detect Occludin in samples from Human, Mouse, Rat and Dog.

Quick Overview for Occludin antibody (C-Term) (FITC) (ABIN204503)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: OC-3F10

Product Details anti-Occludin Antibody (FITC)

Binding Specificity: C-Term

Specificity: Recognizes human Occludin Protein. Species cross-reactivity: mouse, rat and canine.

Purification: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 μg/mL. Western Blot: Detects a band at ~65kD. Immunofluorescence: 2-10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: -20°C
Protect from light.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C