Phospholamban antibody (pSer16)

Catalog No. ABIN204571

This Rabbit Polyclonal antibody specifically detects Phospholamban in ELISA and WB. It exhibits reactivity toward Human and Cow.

Quick Overview for Phospholamban antibody (pSer16) (ABIN204571)

Target: Phospholamban (PLN)

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-Phospholamban Antibody

Binding Specificity: AA 14-25, pSer16

Specificity: Recognizes human Phospholamban phosphorylated (Ser16) (Mr 5-25kD). Species cross-reactivity: bovine. Species sequence Homology: mouse, rat, rabbit, porcine and canine.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa14-25 of human cardiac phospholamban with a cysteine added at the C-terminal (RA[pS]TIEMPQQAR-C) (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. Additional non-specific bands may be detected in some lysates (Mr 75 & 100kD).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLN / Phospholamban

Background: Name/Gene ID: PLN

Synonyms: PLN, CMD1P, CMH18, PLB, Cardiac phospholamban, Phospholamban

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity