WC1 Surface Antigen antibody (FITC)

Catalog No. ABIN206277

The FITC-conjugated Mouse Monoclonal anti-WC1 Surface Antigen antibody (ABIN206277) specifically detects WC1 Surface Antigen in FACS. The antibody is reactive with Cow and Pig samples.

Quick Overview for WC1 Surface Antigen antibody (FITC) (ABIN206277)

Target: WC1 Surface Antigen

Reactivity: Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Product Details

Specificity: Recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform. The bovine WC1 cell surface antigen is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. Immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homolog of WC1, which is a 180 kD molecule. In pigs the 180 kD molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8.

Purification: Protein G purified

Immunogen: Con A stimulated bovine lymphocytes.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:10)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: WC1 Surface Antigen