ZAP70 antibody (C-Term)

Catalog No. ABIN207086

The Rabbit Polyclonal anti-ZAP70 antibody has been validated for WB and Inhibition. It is suitable to detect ZAP70 in samples from Human.

Quick Overview for ZAP70 antibody (C-Term) (ABIN207086)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Blocking Antibody (Inhibition)

Product Details anti-ZAP70 Antibody

Binding Specificity: C-Term

Specificity: Recognizes C-terminal part of human ZAP-70 protein tyrosine kinase

Purification: Protein A purified

Immunogen: Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70.

Type of Immunogen: Fusion protein

Application Details

Application Notes: Approved: Inhb, WB (1 μg/mL)

Usage: Western Blotting: Recommended dilution - 1 μg/mL. Positive control - JURKAT T cell leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl  pH 8.2, 50 mM NaF includ

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway