ZAP70 antibody (C-Term)

Catalog No. ABIN207127

This Mouse Monoclonal antibody specifically detects ZAP70 in WB, FACS, ICC and Inhibition. It exhibits reactivity toward Human.

Quick Overview for ZAP70 antibody (C-Term) (ABIN207127)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Blocking Antibody (Inhibition)

Clone: ZAP-03

Product Details anti-ZAP70 Antibody

Binding Specificity: C-Term

Specificity: Reacts with ZAP-70, a 70 kD protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

Purification: Protein A purified

Immunogen: Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (2 - 5 μg/mL), ICC, Inhb, WB (5 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 2-5 μg/mL. Positive control -. HPB-ALL human peripheral blood T cell leukemia cell line. Application note - Intracellular staining. Western Blotting: Recommended dilution - 0,5 μg/mL. Positive control -. HPB-ALL huma

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway