DNMT3L antibody (AA 152-164)

Catalog No. ABIN208362

This anti-DNMT3L antibody is a Rabbit Polyclonal antibody detecting DNMT3L in WB, ELISA and IP. Suitable for Human.

Quick Overview for DNMT3L antibody (AA 152-164) (ABIN208362)

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-DNMT3L Antibody

Binding Specificity: AA 152-164

Specificity: Recognizes Human DNMT3L MW: 43.6kD. Species sequence Homology: Mouse: 76 % .

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey (92%) Rat, Hamster, Panda, Bat (85%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to aa 152-164, GLLQRRRKWRSQL of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey (92%), Rat, Hamster, Panda, Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:3000), IP, WB (1:1500)

Usage: Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 1:1000-1:3000. Western Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells. (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150  mM. Does not perform well when applied directly for Western Blot.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L

Background: Name/Gene ID: DNMT3L
Family: C5-methyltransferase

Synonyms: DNMT3L

Gene ID: 29947

UniProt: Q9UJW3