MMP14 antibody (AA 319-333)

Catalog No. ABIN209030

The Mouse Monoclonal anti-MMP14 antibody has been validated for WB, ELISA, IHC, IP, IHC (p) and IHC (fro). It is suitable to detect MMP14 in samples from Human, Mouse, Rat, Cow, Monkey, Goat, Hamster and Sheep.

Quick Overview for MMP14 antibody (AA 319-333) (ABIN209030)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Goat, Hamster, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP14 Antibody

Binding Specificity: AA 319-333

Specificity: Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).

Purification: Immunoaffinity purified

Immunogen: Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).

Isotype: IgG3 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 2 % BSA (protease free).

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP14 / MMP-14

Background: Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1

Gene ID: 4323

UniProt: P50281

Pathways: Autophagy