MCL-1 antibody (AA 1-327)

Catalog No. ABIN209035

This Mouse Monoclonal antibody specifically detects MCL-1 in WB, ELISA, IHC, IHC (p), IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MCL-1 antibody (AA 1-327) (ABIN209035)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MCL-1 Antibody

Binding Specificity: AA 1-327

Specificity: Recognizes human Mcl-1 (Myeloid cell leukemia-1) (MW 42kD).

Purification: Protein G purified

Immunogen: Recombinant human Mcl-1 protein corresponding to amino acid 1-327.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P, IP (1:100 - 1:200), WB (1:100 - 1:200)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1:100-1:200. Immunoprecipitation: 10 μL/mg of cell lysate. Immunohistochemistry: (with cryostat or formalin-fixed paraffin embedded sections). Staining of formalin fixed paraffin embedded tissues requires boiling the tissue sections in 10 mM citrate buffer,  pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Western Blot: ML-1 or Raji cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL1 / MCL 1

Background: Name/Gene ID: MCL1

Synonyms: MCL1, BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, Bcl2-L-3, MCL1S, MCL1L, Myeloid cell leukemia ES, MCL 1, MCL1-ES, TM, EAT, Mcl-1

Gene ID: 4170

UniProt: Q07820

Pathways: MAPK Signaling