OCT4 antibody (AA 282-297)
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- Target See all OCT4 (POU5F1) Antibodies
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Binding Specificity
- AA 282-297
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Reactivity
- Human, Rodents
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This OCT4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide (aa 282-297) of Oct4.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product POU5F1 Primary Antibody
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- Application Notes
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Approved: ICC (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:200 - 1:1000)
Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20 °C. Alternative is fixation with 4 % PFA and treatment with 0.05 % trypsin solution (incubation 10 min. at 37 °C, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50 mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4. No preservative added.
- Preservative
- Without preservative
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Alternative Name
- POU5F1 / OCT4 (POU5F1 Products)
- Synonyms
- OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
- Background
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Name/Gene ID: POU5F1
Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3 - Gene ID
- 5460
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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