Phospholipase C gamma 1 antibody

Catalog No. ABIN209160

This anti-Phospholipase C gamma 1 antibody is a Mouse Monoclonal antibody detecting Phospholipase C gamma 1 in WB, ELISA and IP. Suitable for Human, Mouse, Rat, Cow and Rabbit.

Quick Overview for Phospholipase C gamma 1 antibody (ABIN209160)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Phospholipase C gamma 1 Antibody

Specificity: Recognizes bovine PLCg-1 at ~135kD. May also react with some non-PLC g-1 SH-3 containing proteins. Species cross-reactivity: rabbit, rat, mouse and human.

Purification: Protein G purified

Immunogen: Purified soluble phospholipase C (PLC) g-1 from bovine brain.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IP, WB (0.1 - 0.5 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Western Blot: 0.1-0.5 μg/mL. Detects PLCg1 in acid precipitated protein from bovine brain, RIPA lysates from A431 cells, and lysates from 3Y3/A31 fibroblasts and L6 myoblasts. Immunoprecipitation: 4 μg (bovine brain cytosol).

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: dH2O, 40-50 % glycerol

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1

Background: Name/Gene ID: PLCG1
Family: Phospholipase

Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1

Gene ID: 5335

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling