PTTG1 antibody (N-Term)

Catalog No. ABIN209196

Product Details anti-PTTG1 Antibody

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human PTTG-1. Expected to cross-react with mouse PTTG-1 based sequence homology. Reactivity confirmed with human MCF-7 cell lysate (Western Blot) and colon cancer tissue (IHC, paraffin).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the transactivation domain within the N-terminus of the human PTTG-1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (1 - 2 μg/mL), WB (2 - 4 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 2-4 μg/mL. Recognizes only the 28kD band in MCF-7 cell lysate. Immunohistochemistry: 1-2 μg/mL in formalin-fixed, paraffin embedded tissues. For best results heat induced epitope retrieval (HIER) with citrate buffer,  pH 6.0, is required prior to staining.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: Securin / PTTG1 (PTTG1 Products)

Synonyms: EAP1 antibody, HPTTG antibody, PTTG antibody, TUTR1 antibody, PTTG1 antibody, securin antibody, AW555095 antibody, C87862 antibody, Pttg antibody, Pttg3 antibody, Securin antibody, Protein Cut2 antibody, pituitary tumor-transforming 1 antibody, pituitary tumor-transforming gene 1 antibody, securin, sister chromatid separation inhibitor antibody, PTTG1 antibody, Pttg1 antibody, cut2 antibody

Background: Name/Gene ID: PTTG1

Synonyms: PTTG1, EAP1, Esp1-associated protein, HPTTG, ESP1-associated protein 1, Pituitary tumor-transforming 1, TUTR1, PTTG, Securin, Tumor-transforming protein 1

Gene ID: 9232

UniProt: O95997