RAF1 antibody (N-Term)

Catalog No. ABIN209206

This Mouse Monoclonal antibody specifically detects RAF1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for RAF1 antibody (N-Term) (ABIN209206)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RAF1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes Raf-1 N-terminal (aa1-240).

Purification: Protein G purified

Immunogen: Purified recombinant Raf-1 (75kD).

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, WB (1 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Not suitable for the detection of Raf-1 expression and phosphorylation in HeLa cells. Western Blot: 1 μg/mL will allow visualization of 0.2 μg/lane of human Raf-1. A main band (75kD) was visible.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1 / RAF

Background: Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase

Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase

Gene ID: 5894

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling