SOS1 antibody (AA 1243-1258)

Catalog No. ABIN210638

The Rabbit Polyclonal anti-SOS1 antibody is suitable to detect SOS1 in samples from Human, Mouse, Horse, Rabbit, Dog and Monkey. It has been validated for WB and IP.

Quick Overview for SOS1 antibody (AA 1243-1258) (ABIN210638)

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Reactivity: Human, Mouse, Horse, Rabbit, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-SOS1 Antibody

Binding Specificity: AA 1243-1258

Specificity: Recognizes SOS 1, Mr 175kD. Species cross-reactivity: Mouse and human. Other species not tested.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%) Bovine, Bat, Pig (94%) Opossum (88%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 1243-1258 (KKSDHGNAFFPNSPSP) of murine Son of Sevenless Homolog 1 Protein (SOS 1). The immunizing sequence is identical in human. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Horse, Rabbit (100%), Bovine, Bat, Pig (94%), Opossum (88%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 5 μg. Positive control: F4209: Fibroblast Cell, 3T3/A31. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris,  pH 8.0, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SOS1

Target: SOS1 (Son of Sevenless Homolog 1 (SOS1))

Alternative Name: SOS1 / HGF

Background: Name/Gene ID: SOS1

Synonyms: SOS1, GINGF, NS4, SOS-1, Son of sevenless homolog 1, GF1, GGF1

Gene ID: 6654

UniProt: Q07889

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling