ZBTB33 antibody (AA 103-584)

Catalog No. ABIN210906

Product Details anti-ZBTB33 Antibody

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Binding Specificity: AA 103-584

Reactivity: Human, Mouse, Rat, Dog, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBTB33 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Specificity: Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.

Purification: Protein G purified

Immunogen: Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZBTB33

Target: ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

Alternative Name: ZBTB33 / Kaiso (ZBTB33 Products)

Synonyms: kaiso antibody, ZNF-kaiso antibody, ZNF348 antibody, AW260253 antibody, E130014G12Rik antibody, Kaiso antibody, Znf-kaiso antibody, RGD1566309 antibody, zinc finger and BTB domain containing 33 S homeolog antibody, zinc finger and BTB domain containing 33 antibody, zbtb33.S antibody, ZBTB33 antibody, Zbtb33 antibody

Background: Name/Gene ID: ZBTB33

Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348

Gene ID: 10009

UniProt: Q86T24