Lysine (K)-Specific Methyltransferase 2A (KMT2A) (AA 3084-3959) antibody

Catalog No. ABIN210920

The Mouse Monoclonal anti-Lysine (K)-Specific Methyltransferase 2A antibody is suitable to detect Lysine (K)-Specific Methyltransferase 2A in samples from Human and Mouse. It has been validated for WB and IP.

Quick Overview for Lysine (K)-Specific Methyltransferase 2A (KMT2A) (AA 3084-3959) antibody (ABIN210920)

Target: Lysine (K)-Specific Methyltransferase 2A (KMT2A)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 4H354

Product Details

Binding Specificity: AA 3084-3959

Specificity: Recognizes the C-terminal proteolytic fragment of human MLL (MLLC), Mr ~180kD. Species cross-reactivity: Human and mouse.

Purification: Protein G purified

Immunogen: MBP fusion protein corresponding to residues 3084-3959 of human mixed lineage leukemia (MLL).

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate MLLC from nuclear extracts. The N-terminal fragment, MLLN (300kD), is also co-immunoprecipitated by this antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Lysine (K)-Specific Methyltransferase 2A (KMT2A)

Alternative Name: KMT2A / MLL

Background: Name/Gene ID: KMT2A

Synonyms: KMT2A, ALL1, CDK6/MLL fusion protein, FLJ11783, HRX, HTRX, HTRX1, Lysine N-methyltransferase 2A, MLL1A, MLL/GAS7, MLL1, ALL-1, MLL, MLL/GAS7 fusion protein, TRX1, WDSTS, Trithorax-like protein, Zinc finger protein HRX, CXXC7, MLL-AF4 der(11) fusion protein, MLL/GMPS fusion protein, TET1-MLL

Gene ID: 4297

UniProt: Q03164

Pathways: Warburg Effect