REST antibody (AA 801-1097)

Catalog No. ABIN210952

This Rabbit Polyclonal antibody specifically detects REST in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for REST antibody (AA 801-1097) (ABIN210952)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-REST Antibody

Binding Specificity: AA 801-1097

Specificity: Recognizes REST, Mr 120-200kD. Species cross-reactivity: Human, mouse and rat.

Purification: Protein A purified

Immunogen: GST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: Suitable for use in Western Blot. Western Blot Analysis: 0.5-2 μg/mL of this lot detected REST in nuclear extracts from HeLa and Raji cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-REST (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates REST.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: NRSF / REST

Background: Name/Gene ID: REST

Synonyms: REST, X2 box repressor, XBR, NRSF

Gene ID: 5978

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity