SH3KBP1 antibody

Catalog No. ABIN210959

The Mouse Monoclonal anti-SH3KBP1 antibody has been validated for WB, ICC and IP. It is suitable to detect SH3KBP1 in samples from Human and Rat.

Quick Overview for SH3KBP1 antibody (ABIN210959)

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SH3KBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SH3KBP1 Antibody

Specificity: Err:509

Purification: Protein G purified

Immunogen: 6His and HA tagged fusion protein corresponding to full length rat SETA.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ICC, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 μg/mL detects SETA in RIPA lysates from NG108-15 cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes. Immunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SH3KBP1

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Alternative Name: SH3KBP1 / CIN85

Background: Name/Gene ID: SH3KBP1

Synonyms: SH3KBP1, CD2BP3, CIN85, GIG10, HSB-1, HSB1, C-Cbl-interacting protein, CD2-binding protein 3, MIG18, Migration-inducing gene 18

Gene ID: 30011

UniProt: Q96B97

Pathways: EGFR Signaling Pathway, EGFR Downregulation