CSNK2A1/CK II alpha antibody (AA 7-22)

Catalog No. ABIN211082

The Rabbit Polyclonal anti-CSNK2A1/CK II alpha antibody has been validated for WB and ELISA. It is suitable to detect CSNK2A1/CK II alpha in samples from Human, Mouse, Rat, Cow, Dog, Chicken, Horse, Pig, Xenopus laevis, Bat, Monkey and Hamster.

Quick Overview for CSNK2A1/CK II alpha antibody (AA 7-22) (ABIN211082)

Target: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Horse, Pig, Xenopus laevis, Bat, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSNK2A1/CK II alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CSNK2A1/CK II alpha Antibody

Binding Specificity: AA 7-22

Specificity: Recognizes human casein kinase 2 at 44kD and an unknown band at 66kD. Species cross-reactivity: Mouse. Predicted cross-reactivity with rat, rabbit, chicken, frog and starfish.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Xenopus (100%) Rabbit, Platypus, Stickleback, Pufferfish, Seq squirt (94%) Zebrafish, Sea anemone, Trichoplax, Capsaspora (88%) Salmon, Ant, Beetle (81%).

Purification: Protein A purified

Immunogen: Synthetic peptide (C-SRARVYTDVNTHRPRE) corresponding to amino acids 7-22 of human Casein Kinase 2, KLH-conjugated. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Xenopus (100%), Rabbit, Platypus, Stickleback, Pufferfish, Seq squirt (94%), Zebrafish, Sea anemone, Trichoplax, Capsaspora (88%), Salmon, Ant, Beetle (81%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1 - 4 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Not suitable for use in Immunoprecipitation. Western Blot: 1-4 μg/mL will detect Casein Kinase 2 in RIPA lysates from mouse 3T3 fibroblasts. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Casein Kinase 2 (4 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CSNK2A1/CK II alpha

Target: CSNK2A1/CK II alpha (CSNK2A1) (Casein Kinase 2 alpha 1 (CSNK2A1))

Alternative Name: CSNK2A1 / CKII

Background: Name/Gene ID: CSNK2A1
Subfamily: Casein kinase II
Family: Protein Kinase

Synonyms: CSNK2A1, Casein kinase II alpha, Casein kinase II subunit alpha, Ck2 alpha, CK2 catalytic subunit alpha, CKII, CKIIalpha, CK2A1, CKII alpha, Protein kinase CK2, Casein kinase 2 alpha, CK II alpha

Gene ID: 1457

UniProt: P68400

Pathways: SARS-CoV-2 Protein Interactome