NG2 antibody (AA 1592-2222)

Catalog No. ABIN211128

The Mouse Monoclonal anti-NG2 antibody (ABIN211128) specifically detects NG2 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for NG2 antibody (AA 1592-2222) (ABIN211128)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NG2 Antibody

Binding Specificity: AA 1592-2222

Specificity: Recognizes NG2, Mr ~270-300kD. Species cross-reactivity: Rat. Species sequence homology: mouse.

Purification: Protein G purified

Immunogen: HEK293 cells expressing a truncated integral membrane form of NG2 consisting of aa1592-2222.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: IHC (1 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 1 μg/mL, Reported to immunostain 4 % paraformaldehyde fixed adult rat brain sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4 / NG2

Background: Name/Gene ID: CSPG4

Synonyms: CSPG4, MCSP, MEL-CSPG, MSK16, NG2, HMW-MAA, MCSPG

Gene ID: 1464

UniProt: Q6UVK1

Pathways: Glycosaminoglycan Metabolic Process