CREB1 antibody (pSer133)

Catalog No. ABIN211133

The Mouse Monoclonal anti-CREB1 antibody has been validated for WB. It is suitable to detect CREB1 in samples from Human, Mouse and Rat.

Quick Overview for CREB1 antibody (pSer133) (ABIN211133)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Specificity: Recognizes phospho-CREB (Ser133), Mr 43kD. Species cross-reactivity: Rat and mouse. Broad species cross-reactivity expected, based on sequence homology.

Purification: Protein G purified

Immunogen: Proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000 to 1:4000. Detects phospho-CREB (Ser133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Lysates from untreated (Lanes 1 and 3) and forskolin treated (Lanes 2 and 4) PC-12 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (Ser133) (1:4000 dilution, Lanes 1 and 2) or anti-CREB (1:2000 dilution, Lanes 3 and 4). Proteins were visualized using a goat antimouse (Lanes 1and 2) or goat antirabbit (Lanes 3 and 4) secondary antibody conjugated to HRP and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1 / CREB

Background: Name/Gene ID: CREB1
Family: Transcription factor

Synonyms: CREB1, CREB-1, Transactivator protein, CREB

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation