Cyclin B1 antibody

Catalog No. ABIN211136

The Mouse Monoclonal anti-Cyclin B1 antibody has been validated for WB and IP. It is suitable to detect Cyclin B1 in samples from Human.

Quick Overview for Cyclin B1 antibody (ABIN211136)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Cyclin B1 Antibody

Specificity: Specific for human cyclin B1 (~58kD). Does not cross-react with cyclin A or cyclin D. Species cross-reactivity: Human, weak species cross-reactivity with mouse was observed.

Purification: Protein G purified

Immunogen: Recombinant human cyclin B1.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IP, WB (0.5 - 5 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Recommended Dilution. Western Blot: 0.5-5 μg/mL detects cyclin B1 in RIPA lysates from human A431 carcinoma cells. Immunoprecipitation: 2 μg precipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1 / Cyclin B1

Background: Name/Gene ID: CCNB1

Synonyms: CCNB1, CCNB, Cyclin B1, G2/mitotic-specific cyclin-B1, G2/mitotic-specific cyclin B1

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase