NCK2 antibody (AA 175-190)

Catalog No. ABIN211251

This anti-NCK2 antibody is a Rabbit Polyclonal antibody detecting NCK2 in WB and IP. Suitable for Mouse and Rat.

Quick Overview for NCK2 antibody (AA 175-190) (ABIN211251)

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-NCK2 Antibody

Binding Specificity: AA 175-190

Specificity: Recognizes mouse Nck beta, Mr 47kD. Species sequence homology: human.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (94%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to aa175-190 of murine Nck beta [EAPSFLSLRRGTALSN] (KLH-conjugated). Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (94%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:100), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 1:100. Immunoprecipitates. Nck beta from 1-2 mg of mouse tissue homogenates.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Alternative Name: GRB4 / NCK2

Background: Name/Gene ID: NCK2

Synonyms: NCK2, GRB4, NCKbeta, NCK beta, Nck-2, Cytoplasmic protein NCK2, NCK adaptor protein 2

Gene ID: 8440

UniProt: O43639

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization