NCK2 antibody (AA 300-380)

Catalog No. ABIN7225414

The Rabbit Polyclonal anti-NCK2 antibody is suitable to detect NCK2 in samples from Human and Mouse. It has been validated for IHC, ELISA and IF.

Quick Overview for NCK2 antibody (AA 300-380) (ABIN7225414)

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NCK2 Antibody

Binding Specificity: AA 300-380

Purpose: Rabbit Anti-Nck-2 Polyclonal Antibody

Specificity: Nck-2 Polyclonal Antibody detects endogenous levels of Nck-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Nck-2 at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Alternative Name: Nck-2

Background: NCK2, GRB4, Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, Nck-2, SH2/SH3 adaptor protein NCK-betaNCK2 (NCK adaptor protein 2) encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene ID: 8440

UniProt: O43639

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization