MPL antibody (AA 615-628)

Catalog No. ABIN211285

This Rabbit Polyclonal antibody specifically detects MPL in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Hamster and Monkey.

Quick Overview for MPL antibody (AA 615-628) (ABIN211285)

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Reactivity: Human, Mouse, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-MPL Antibody

Binding Specificity: AA 615-628

Specificity: Recognizes glycosylated and unglycosylated human TPO receptor/c-Mpl, Mr 70kD. Species cross-reactivity: mouse. Species sequence Homology: mouse - 100 % .

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster (100%) Elephant, Rabbit (93%) Panda, Bovine, Pig (86%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to amino acids 615-628 of human TPO (thrombopoietin) receptor [C-ANHSYLPLSYWQQP] (KLH-conjugated). Species sequence Homology: Mouse - 100%. Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster (100%), Elephant, Rabbit (93%), Panda, Bovine, Pig (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IP, WB (0.25 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Stains megakaryocytes in bone marrow biopsy sections. Immunoprecipitation: 5 μg immunoprecipitates TPO receptor from 500 μg of DAMI RIPA lysate.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MPL

Target: MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))

Alternative Name: CD110 / MPL

Background: Name/Gene ID: MPL

Synonyms: MPL, CD110 antigen, C-MPL, CD110, MPLV, Proto-oncogene c-Mpl, TPOR, TPO-R, THCYT2, Thrombopoietin receptor

Gene ID: 4352

UniProt: P40238

Pathways: JAK-STAT Signaling