ACTN1 antibody

Catalog No. ABIN211332

The Mouse Monoclonal anti-ACTN1 antibody (ABIN211332) specifically detects ACTN1 in WB, IHC, ICC, IP and IHC (fro). The antibody is reactive with Human, Mouse and Rabbit samples.

Quick Overview for ACTN1 antibody (ABIN211332)

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human, Mouse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ACTN1 Antibody

Specificity: Recognizes human alpha-actinin 1 isoform (non-muscle) at 100kD. Species cross-reactivity: Mouse and rabbit. Species sequence homology: Human alpha actinin 2 - 77 % (muscle, myocardium).

Purification: Protein A purified

Immunogen: Human alpha-actinin.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Western Blot: 0.5-2 μg/mL. Detects alpha-Actinin in RIPA lysates from human A431 cells. Immunocytochemistry/Immunohistochemistry: 15 μg/mL. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: ACTN1

Background: Name/Gene ID: ACTN1

Synonyms: ACTN1, Alpha-actinin-1, Actinin 1 smooth muscle, Actinin, alpha 1, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ASMA

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization