Vinculin antibody

Catalog No. ABIN211429

This Mouse Monoclonal antibody specifically detects Vinculin in WB, IHC, IP, ICC and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Chicken.

Quick Overview for Vinculin antibody (ABIN211429)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Rabbit, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Vinculin Antibody

Specificity: Recognizes human vinculin at 117kD. Species cross-reactivity: rat, mouse, rabbit and chicken.

Purification: Protein A purified

Immunogen: Human vinculin.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10 μg/mL will stain Vinculin in Ref-52 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL / Vinculin

Background: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gene ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2