EGFR antibody (Extracellular Domain)

Catalog No. ABIN211610

The Rat Monoclonal anti-EGFR antibody has been validated for IHC, FACS, IP and IHC (fro). It is suitable to detect EGFR in samples from Human.

Quick Overview for EGFR antibody (Extracellular Domain) (ABIN211610)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ICR10

Product Details anti-EGFR Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes the human epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. Binds to epitope B from EGFR and has an affinity of 6.7 x 10-9 M.

Purification: Ion exchange chromatography

Immunogen: Extracellular domain of human EGF-receptor from head and neck carcinoma.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Not recommended for: IHC-P, WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Gene ID: 1956

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins