Annexin a1 antibody (AA 1-346)

Catalog No. ABIN2116708

This Mouse Monoclonal antibody specifically detects Annexin a1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Annexin a1 antibody (AA 1-346) (ABIN2116708)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: D3

Product Details anti-Annexin a1 Antibody

Binding Specificity: AA 1-346

Purpose: Monoclonal Antibody to Annexin A1 (ANXA1)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MAMVSEFLKQ ACYIEKQEQE YVQAVKSYKG GPGSAVSPYP SFNPSSDVAA LHKAIMVKGV DEATIIDILT KRTNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA MLKTPAQFDA DELRAAMKGL GTDEDTLIEI LTTRSNQQIR EITRVYREEL KRDLAKDITS DTSGDFRNAL LALAKGDRCE DMSVNQDLAD TDARALYEAG ERRKGTDVNV FNTILTTRSY PHLRKVFQNY RKYSQHDMNK ALDLELKGDI EKCLTTIVKC ATSTPAFFAE KLYEAMKGAG TRHKTLIRIM VSRSEIDMNE IKVFYQKKYG IPLCQAILDE TKGDYEKILV ALCGGN

Specificity: The antibody is a mouse monoclonal antibody raised against ANXA1. It has been selected for its ability to recognize ANXA1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPE787Ra01Recombinant Annexin A1 (ANXA1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: ANXA1

Background: ANX-A1, ANX1, LPC1, Lipocortin I, Chromobindin-9, Calpactin II, Phospholipase A2 inhibitory protein

Pathways: Hormone Transport