ICAM1 antibody

Catalog No. ABIN7472991

This anti-ICAM1 antibody is a Mouse Monoclonal antibody detecting ICAM1 in WB, FACS, IHC, IP, IHC (p) and IHC (fro). Suitable for Rat.

Quick Overview for ICAM1 antibody (ABIN7472991)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ICAM1 Antibody

Specificity: Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.

Purification: Protein G purified

Immunogen: Rat Ax cells (a HEV derived cell line).

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:25 - 1:50), IHC, IHC-Fr, IHC-P, IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1 / CD54

Background: Name/Gene ID: ICAM1
Family: ICAM

Synonyms: ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58

Gene ID: 3383

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling