Acetylcholinesterase antibody (AA 377-574)

Catalog No. ABIN2119069

This Mouse Monoclonal antibody specifically detects Acetylcholinesterase in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Acetylcholinesterase antibody (AA 377-574) (ABIN2119069)

Target: Acetylcholinesterase (AChE)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: C1

Product Details anti-Acetylcholinesterase Antibody

Binding Specificity: AA 377-574

Purpose: Monoclonal Antibody to Acetylcholinesterase (ACHE)

Sequence: MGHHHHHHSG SEF- FSKD NESLISRAEF LAGVRVGVPQ VSDLAAEAVV LHYTDWLHPE DPARLREALS DVVGDHNVVC PVAQLAGRLA AQGARVYAYV FEHRASTLSW PLWMGVPHGY EIEFIFGIPL DPSRNYTAEE KIFAQRLMRY WANFARTGDP NEPRDPKAPQ WPPYTAGAQQ YVSLDLRPLE VRRGLRAQAC AFWNRFLPKL LSAT

Specificity: The antibody is a mouse monoclonal antibody raised against ACHE. It has been selected for its ability to recognize ACHE in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPB447Hu01Recombinant Acetylcholinesterase (ACHE)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: ACHE

Background: ARAChE, N-AChE, YT

Pathways: Skeletal Muscle Fiber Development