GAPDH antibody

Catalog No. ABIN211932

The Mouse Monoclonal anti-GAPDH antibody (ABIN211932) specifically detects GAPDH in WB, ELISA, IF and ICC. The antibody is reactive with Human, Mouse, Rat, Chicken, Rabbit and Frog samples.

Quick Overview for GAPDH antibody (ABIN211932)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GAPDH Antibody

Specificity: Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle. Also recognizes cardiac GAPDH. Species cross-reactivity: human, rat, mouse, chicken, frog and fish skeletal muscle. Does not react with GAPDH from E. coli. GAPDH enzyme is also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.

Purification: Protein G purified

Immunogen: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:1000000), ICC (1:100 - 1:300), IF, WB (1:500)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. ELISA: 1:1000000. Western Blot: 1:500. Detects on 10 μg of A431 lysates. Immunocytochemistry: 1:100-1:300. Dilute in PBS, 10 mg/mL BSA. Fixative: 4 % PFA, 0.02 % PBS-Triton X-100. Incubation time: 3 min at RT.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. glycerol-free.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

UniProt: P04406