GAPDH antibody

Catalog No. ABIN211932

This anti-GAPDH antibody is a Mouse Monoclonal antibody detecting GAPDH in WB, ELISA, IF and ICC. Suitable for Human, Mouse, Rat, Rabbit, Chicken and Frog.

Quick Overview for GAPDH antibody (ABIN211932)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GAPDH Antibody

Specificity: Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle. Also recognizes cardiac GAPDH. Species cross-reactivity: human, rat, mouse, chicken, frog and fish skeletal muscle. Does not react with GAPDH from E. coli. GAPDH enzyme is also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.

Purification: Protein G purified

Immunogen: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:1000000), ICC (1:100 - 1:300), IF, WB (1:500)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. ELISA: 1:1000000. Western Blot: 1:500. Detects on 10 μg of A431 lysates. Immunocytochemistry: 1:100-1:300. Dilute in PBS, 10 mg/mL BSA. Fixative: 4 % PFA, 0.02 % PBS-Triton X-100. Incubation time: 3 min at RT.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. glycerol-free.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

UniProt: P04406