PML antibody (AA 1-581)

Catalog No. ABIN212004

This anti-PML antibody is a Mouse Monoclonal antibody detecting PML in WB and ICC. Suitable for Mouse.

Quick Overview for PML antibody (AA 1-581) (ABIN212004)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-PML Antibody

Binding Specificity: AA 1-581

Specificity: Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Purification: Protein G purified

Immunogen: 6His fusion protein corresponding to aa1-581 of mouse PML (promyelocytic leukemia protein).

Type of Immunogen: Fusion protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ICC, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 μg/mL of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton X-100.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background: Name/Gene ID: PML
Family: Transcription factor

Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71

Gene ID: 5371

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus