PML antibody (C-Term)

Catalog No. ABIN2777721

The Rabbit Polyclonal anti-PML antibody (ABIN2777721) specifically detects PML in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Pig and Rabbit samples.

Quick Overview for PML antibody (C-Term) (ABIN2777721)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PML Antibody

Binding Specificity: C-Term

Purpose: PML Antibody - C-terminal region

Sequence: EGPSTLRVLD ENLADPQAED RPLVFFDLKI DNETQKISQL AAVNRESKFR

Specificity: Isoform 1, 9, 10 and 11 specific

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 85%, Horse: 85%, Human: 100%, Mouse: 100%, Pig: 92%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PML. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PML

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33048-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background:

Background Information: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus.

Gene Name: Promyelocytic leukemia

Alternative Symbols: MYL, RNF71, PP8675, TRIM19

Protein Name: Protein PML

Molecular Weight: 59kDa

Gene ID: 5371

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus