PRMT1 antibody (AA 298-318)
Quick Overview for PRMT1 antibody (AA 298-318) (ABIN212007)
Target
See all PRMT1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 298-318
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Specificity
- Recognize the PRMT1 doublet, Mr ~42-44kD. A non-specific protein was also detected, Mr ~120kD. Species cross-reactivity: Human and mouse.
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Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Dog, Bovine, Horse, Rabbit, Opossum (100%) Xenopus (95%) Monkey (90%) Zebrafish, Stickleback, Pufferfish (86%) Salmon (81%).
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Purification
- Protein A purified
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Immunogen
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KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Dog, Bovine, Horse, Rabbit, Opossum (100%), Xenopus (95%), Monkey (90%), Zebrafish, Stickleback, Pufferfish (86%), Salmon (81%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
Isotype
- IgG
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Application Notes
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Approved: WB
Usage: Suitable for use in Western Blot. Western Blot Analysis: 0.1-0.5 μg/mL of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))
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Alternative Name
- PRMT1
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Background
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Name/Gene ID: PRMT1
Synonyms: PRMT1, HRMT1L2, HMT2, ANM1, HCP1, IR1B4 -
Gene ID
- 3276
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UniProt
- Q99873
Target
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