PRMT5 antibody (AA 60-79)

Catalog No. ABIN7471320

This Rabbit Polyclonal antibody specifically detects PRMT5 in WB and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Rabbit, Monkey and Bat.

Quick Overview for PRMT5 antibody (AA 60-79) (ABIN7471320)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PRMT5 Antibody

Binding Specificity: AA 60-79

Specificity: Recognizes PRMT5, Mr ~70kD. Species cross-reactivity: Human and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%) Xenopus, Bovine (95%) Chicken, Opossum (90%) Zebrafish, Pufferfish (85%) Medaka (80%).

Purification: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to aa60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%), Xenopus, Bovine (95%), Chicken, Opossum (90%), Zebrafish, Pufferfish (85%), Medaka (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5

Background: Name/Gene ID: PRMT5

Synonyms: PRMT5, 72 kDa ICln-binding protein, HRMT1L5, JBP1, Jak-binding protein 1, IBP72, SKB1Hs, SKB1, SKB1 homolog, Skb1 (S. pombe) homolog, SKB1 homolog (S. pombe)

Gene ID: 10419

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development