Paxillin antibody

Catalog No. ABIN212824

This Mouse Monoclonal antibody specifically detects Paxillin in WB, ELISA, IP and ICC. It exhibits reactivity toward Human, Mouse, Rat, Chicken and Cow.

Quick Overview for Paxillin antibody (ABIN212824)

Target: Paxillin (PXN)

Reactivity: Human, Mouse, Rat, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Paxillin Antibody

Specificity: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.

Purification: Protein G purified

Immunogen: GST fusion protein containing full-length human paxillin.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: PXN / Paxillin

Background: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

Gene ID: 5829

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling