ERBB3 antibody (C-Term)

Catalog No. ABIN213195

This anti-ERBB3 antibody is a Rabbit Polyclonal antibody detecting ERBB3 in WB and IP. Suitable for Human, Mouse and Rat.

Quick Overview for ERBB3 antibody (C-Term) (ABIN213195)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ERBB3 Antibody

Binding Specificity: C-Term

Specificity: Recognizes human HER3 at 180kD. Species cross-reactivity: mouse and rat. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum.

Purification: Protein A purified

Immunogen: A GST-HER3 fusion protein corresponding to aa1283 to 1323 at the carboxy-terminus of human HER3.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 5 μL. Use 25 μL of Protein A-agarose beads and 1 mL of lysate (lysate contains approximately 1 mg of total protein). Rotate mixture overnight at 4?C and then wash 3x with lysis buffer. Dissolve the resulting bead complex in 20-30 μL of 3xSDS-PAGE sample buffer and load ~15 μL per lane on an 8 % polyacrylamide gel. Positive control: Human prostate cell lines (LNCap) or human breast cell line (MDA-453).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: HER3 / ERBB3

Background: Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

Gene ID: 2065

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway