GPR3 antibody (Extracellular Domain)

Catalog No. ABIN213338

The Rabbit Polyclonal anti-GPR3 antibody has been validated for ELISA, IHC and IHC (p). It is suitable to detect GPR3 in samples from Human, Monkey, Cow and Rabbit.

Quick Overview for GPR3 antibody (Extracellular Domain) (ABIN213338)

Target: GPR3 (G Protein-Coupled Receptor 3 (GPR3))

Reactivity: Human, Monkey, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR3 Antibody

Binding Specificity: Extracellular Domain

Brand: IHC-plus™

Specificity: Human GPR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TKT (44 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Elephant, Panda, Rabbit (100%) Rat, Pig (94%) Mouse, Hamster, Horse (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Elephant, Panda, Rabbit (100%), Rat, Pig (94%), Mouse, Hamster, Horse (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (40 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 40 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPR3

Target: GPR3 (G Protein-Coupled Receptor 3 (GPR3))

Alternative Name: GPR3

Background: Name/Gene ID: GPR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: GPR3, ACCA, ACCA orphan receptor, G protein-coupled receptor 3, GPCR3, Hacca, G-protein coupled receptor 3

Gene ID: 2827

Pathways: cAMP Metabolic Process