PYGM antibody (C-Term)

Catalog No. ABIN213350

The Rabbit Polyclonal anti-PYGM antibody is suitable to detect PYGM in samples from Human. It has been validated for IHC and IHC (p).

Quick Overview for PYGM antibody (C-Term) (ABIN213350)

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PYGM Antibody

Binding Specificity: C-Term

Specificity: Human PYGM / Phosphorylase b. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RARRES1 (57 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey (100%) Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Pig, Armadillo (93%) Dog, Panda, Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 14 amino acid peptide from C-terminus of human PYGM / Phosphorylase b. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey (100%), Galago, Marmoset, Mouse, Rat, Hamster, Rabbit, Pig, Armadillo (93%), Dog, Panda, Horse (86%).

Type of Immunogen: Synthetic phospho-peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PYGM

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Alternative Name: PYGM

Background: Name/Gene ID: PYGM

Synonyms: PYGM, GPMM, Myophosphorylase, Phosphorylase, glycogen muscle

Gene ID: 5837

Pathways: Cellular Glucan Metabolic Process