Serotonin Receptor 5A antibody (Extracellular Domain)

Catalog No. ABIN213372

This anti-Serotonin Receptor 5A antibody is a Rabbit Polyclonal antibody detecting Serotonin Receptor 5A in ELISA, IHC and IHC (p). Suitable for Human, Mouse, Rat, Guinea Pig, Horse, Cow, Dog and Monkey.

Quick Overview for Serotonin Receptor 5A antibody (Extracellular Domain) (ABIN213372)

Target: Serotonin Receptor 5A (HTR5A)

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Cow, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 5A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 5A Antibody

Binding Specificity: Extracellular Domain

Brand: IHC-plus™

Specificity: Human 5HT5A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ABCB6 (56 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Elephant, Panda, Horse, Guinea pig (100%) Opossum (94%) Turkey, Chicken (89%) Lizard, Zebrafish, Stickleback (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 1st extracellular domain of human 5HT5A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Elephant, Panda, Horse, Guinea pig (100%), Opossum (94%), Turkey, Chicken (89%), Lizard, Zebrafish, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Alternative Name: HTR5A / 5-HT5A Receptor

Background: Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A

Gene ID: 3361

Pathways: JAK-STAT Signaling