THRA antibody (Ligand Binding Domain)

Catalog No. ABIN213505

This Rabbit Polyclonal antibody specifically detects THRA in IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for THRA antibody (Ligand Binding Domain) (ABIN213505)

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-THRA Antibody

Binding Specificity: Ligand Binding Domain

Brand: IHC-plus™

Specificity: Human THRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Sheep, Dog, Bovine, Hamster, Elephant, Panda, Rabbit, Horse, Pig (95%) Lizard (85%) Opossum (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from ligand-binding domain of human THRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Sheep, Dog, Bovine, Hamster, Elephant, Panda, Rabbit, Horse, Pig (95%), Lizard (85%), Opossum (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 - 15 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA / THR Alpha

Background: Name/Gene ID: THRA
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: THRA, AR7, C-ERBA-1, C-erbaalpha, EAR-7.1/EAR-7.2, ERB-T-1, ERBA1, EAR-7, ERBA-related 7, HTR alpha 1, HTR alpha 2, THRA1, Thyroid hormone receptor alpha, T3R, Tralpha2, Triiodothyronine receptor, V-erbA-related protein 7, Thyroid hormone receptor, Tralpha1, NR1A1, THRA3, C-erbA-alpha, CHNG6, EAR7, ERBA, Erba-alpha, THRA2, TR alpha, Tralpha

Gene ID: 7067

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development