PKC zeta antibody (N-Term)

Catalog No. ABIN213577

This Rabbit Polyclonal antibody specifically detects PKC zeta in ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PKC zeta antibody (N-Term) (ABIN213577)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC zeta antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC zeta Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human PRKCZ / PKC-Zeta. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (95%) Marmoset, Mouse, Rat, Rabbit (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from near N-terminus of human PRKCZ / PKC-Zeta. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey (95%), Marmoset, Mouse, Rat, Rabbit (89%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Alternative Name: PRKCZ / PKC-zeta

Background: Name/Gene ID: PRKCZ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta

Gene ID: 5590

Pathways: NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling