IL-25 antibody (AA 115-132)

Catalog No. ABIN213801

The Mouse Monoclonal anti-IL-25 antibody (Clone 68C1039-2) (ABIN213801) specifically detects IL-25 in WB, IHC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for IL-25 antibody (AA 115-132) (ABIN213801)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 68C1039-2

Product Details anti-IL-25 Antibody

Binding Specificity: AA 115-132

Specificity: A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen.

Purification: Protein G purified

Immunogen: Synthetic peptide from human IL25 / IL-25.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS with 0.05 % sodium azide. Sourced from tissue culture supernatant.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL25 / IL17E

Background: Name/Gene ID: IL25
Family: Interleukin

Synonyms: IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E

Gene ID: 64806

UniProt: Q9H293