NEK2 antibody (AA 287-299)

Catalog No. ABIN213864

Product Details anti-NEK2 Antibody

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Binding Specificity: AA 287-299

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Aa 287-299 of Human NEK2 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human NEK2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2 (NEK2 Products)

Synonyms: AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody

Background: Name/Gene ID: NEK2
Subfamily: NEK
Family: Protein Kinase

Synonyms: NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2

Gene ID: 4751

UniProt: P51955

Pathways: M Phase