IRF3 antibody (C-Term)

Catalog No. ABIN213923

The Rabbit Polyclonal anti-IRF3 antibody has been validated for WB, IHC, IF and IHC (p). It is suitable to detect IRF3 in samples from Human and Mouse.

Quick Overview for IRF3 antibody (C-Term) (ABIN213923)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRF3 Antibody

Binding Specificity: C-Term

Specificity: At least three isoforms of IRF3 are known to exist.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (20 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3 / IRF-3

Background: Name/Gene ID: IRF3

Synonyms: IRF3, Interferon regulatory factor 3, IRF-3

Gene ID: 3661

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades