Fibronectin antibody

Catalog No. ABIN2141941

The Rabbit Polyclonal anti-Fibronectin antibody is suitable to detect Fibronectin in samples from Human. It has been validated for WB, IHC, ELISA and IP.

Quick Overview for Fibronectin antibody (ABIN2141941)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-Fibronectin Antibody

Specificity: This antibody is specific for Human Factor XIII.

Cross-Reactivity (Details): This antibody reacts with Human Fibronectin. Ths antibody has negligible cross reactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml

Purification: Affinity chromatography purified

Sterility: Sterile filtered

Immunogen: Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid In 0.02 M K3PO4, pH 7.2, with 0.15 M NaCl, and 0.01 % Sodium azide.

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Fibronectin

Target: Fibronectin

Background: Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.